Figure 5.

Expression levels of genes in the hypothalamus of p62^−/− mice compared with wild-type controls. The relative expression levels of Pomc, Cart, Npy, Agrp, ObRb, and Socs3 mRNAs in the hypothalamus under ad libitum feeding conditions or after fasting for 48 h in body weight-matched wild-type (blue, 27.6 ± 0.9 g) and p62^−/− (red, 28.4 ± 1.2 g) mice were measured by quantitative real-time PCR. Each sample was assayed in triplicate and normalized to cyclophilin B (9–11-weeks-old; mean ± SD; n = 5–7). Asterisks represent statistical significance compared with each ad libitum-fed control; ***p < 0.001, **p < 0.01, *p < 0.05.