KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti C-peptide | DSHB | Cat#GN-ID4 |
| Rabbit anti Chga | Abcam | Cat#Ab15160 |
| Rabbit anti Cldn4 | Lifespan BioSciences | Cat#LS-B8382 |
| Mouse anti E-cadherin | BD Transduction Laboratories | Cat#610181 |
| Chicken anti GFP | Aves Labs inc. | Cat#GFP-1020 |
| Goat anti glp2 | Santa Cruz Biotechnology | Cat#Sc-7781 |
| Guinea pig anti Insulin | DAKO | Cat#A0564 |
| Hamster anti Muc1 | NeoMarkers | Cat#HM-1630-P1 |
| Rabbit anti Ncad | Invitrogen | Cat#MA5–16324 |
| Goat anti Neurod1 | R&D systems | Cat#AF2746 |
| Rabbit anti Neurogenin3 | EMD Millipore | Cat#Ab10535 |
| Mouse anti Nkx6.1 | DSHB | Cat#F55A12 |
| Rabbit anti proglucagon | Cell Signaling Technology | Cat#D16G10 |
| Rabbit anti Slug | Cell Signaling Technology | Cat#95855 |
| Rabbit anti Sox9 | Abcam | Cat#Ab-5535 |
| Goat anti somatostatin | Santa Cruz Biotechnology | Cat#Sc-7819 |
| Rabbit anti Synaptophysin | ProteinTech Group | Cat#17785–1-AP |
| Chicken anti Vimentin | Encor Biotechnologies | Cat#CPCA-Vim |
| Mouse anti Zeb1 | eBioscience | Cat#14–9741-82 |
| Rabbit anti αSMA | Abcam | Cat#Ab5694 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ActivinA | R&D Systems | Cat#338-AC |
| Rock Inhibitor Y-27632 | DNSK | Cat#DNSK-Kl15–02 |
| Critical Commercial Assays | ||
| ViewRNA ISH Tissue Assay Kit (2-plex) | ThermoFisher | Cat#QVT0012 |
| SMARTScribe Reverse Transcriptase | Clontech | Cat#639537 |
| Advantage2 Polymerase mix | Clontech | Cat#639202 |
| Agencourt Rnaclean | ThermoFisher | Cat#NC0068576 |
| Agencourt AMPure | Beckman Coulter | Cat#A63881 |
| Direct-zol | ThermoFisher | Cat#50444622 |
| Deposited Data | ||
| Raw and analyzed single cell sequencing data | This paper | GEO: GSE121416 |
| Raw and analyzed bulk sequencing data | This paper | GEO: GSE121679 |
| Experimental Models: Cell Lines | ||
| HUES8 hESCS | HSCI | hES Cell line: HUES-8 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Neurog3tm1(EGFP)Khk | Raised in house | RRID:MMRRC_000344-UCD |
| Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl | Charles River | RRID:IMSR_CRL:250 |
| Software and Algorithms | ||
| Houdini | Side Effects Software | www.sidefx.com |
| Topic modeling | This paper | http://groups.csail.mit.edu/cgs/geneprogram.html |
| Monocle | Trapnell lab | http://cole-trapnell-lab.github.io/monocle-release/ |