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In vitro application of the recombinant protein sensor ΔCD−ΔCSD–mEGFP. Relative ID (RID) (A) and signal to noise ratio (SNR) (B) are plotted against protein concentrations in HEK293T NCEs. (A) Inset shows the linear detection range. (C) Images of fixed HEK293T cells stained with recombinant ΔCD−ΔCSD–mEGFP.
