Figure 5.

Fibroblasts increased differentially the radiation long term survival of prostate and breast cancer cells in indirect and 3D direct co-culture. MPR31.4 or Py8119 cancer cells were plated alone or in co-culture (transwell) with stromal fibroblasts for colony formation assay, irradiated with indicated doses (0–10 Gy) and subsequently further incubated for additional 7 days (A–F). Graphs depict the surviving fractions from one representative experiment measured in sextuplet of two independent experiments (means ± SD) (A,B,D,E). The plates were scanned and colonies were counted (C,F). ^*p < 0.5 by one-way ANOVA test followed by Tukey's test. MPR31.4 or Py8119 cancer cells were plated for colony formation assay alone or with NIH-3T3 fibroblasts [ratio (1+1)] in a 3D Matrigel system, irradiated with indicated doses (0–9 Gy) and subsequently further incubated for additional 7 days (G–M). Surviving fractions from three experiments measured in quintuplet each were shown (means ± SEM) (G,J) the colonies were counted and the survival fraction calculated (H,K). Picture of the well-taken in bright light at 2.5 magnifications (I,L). Representative pictures of the well taken by fluorescence microscopy at 10 magnifications (M). ^*p < 0.5, by two-way ANOVA test followed by Tukey's test.