Figure 6.

Fibroblasts did not express a CAFs-like phenotype 72 h after radiation in indirect co-culture with cancer cells. L929 or NIH-3T3 stromal fibroblasts alone (A–C) or together with MPR31.4 or Py8119 cancer cells [transwell, (ratio 1+1)] were cultured 24 h prior irradiation with 0 (D–F) or 10Gy (G–I). After 72 h, qRT-PCR analysis of the CAFs markers α-SMA (Acta2), PDGFR-β and NG2 were performed in total RNA isolates of cultured fibroblasts. Respective expression levels were normalized to β-actin (set at 1). Shown are mean values ± SEM from 3 independent samples per group measured each in triplicate each. “ns” present for no significant and ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 analyzed by one-way ANOVA test followed by Tukey's test.