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Effects of different concentrations of ferric chloride on the growth of Salmonella Typhimurium. (a) In vitro growth of the wild type, (b) in vitro growth of mutant tonB–, and (c) in vitro growth of mutant iroN– fepA–. The interval of each measurement was 15 min for a total of 8 h. The concentrations of ferric chloride supplemented to the IMDM medium ranged from 0 to 10 μM. Values are means ± SEM; n = 5. Control, no iron added.
