Figure 7.

Dynamin and actin are not involved in display exocytosis. A, Effect of dynasore (80 μm) on display and burst event frequency (n = 6 cells). B, Proportion of display events before and during dynasore application. C, Average normalized fluorescence sorted into display (light green and red curves) and burst (dark green and red curves) events for cells transfected with TfR–SEP and dynamin2–mCherry (1061 burst and 823 display events in 9 cells). D, E, Effect of latrunculin A (10 μm, incubated for 20 min before recording) on the total frequency of events (D) and proportion of display events (E). There was no significant difference in either parameter measured in 13 control (Ctrl) and 15 cells treated with latrunculin A (LatA).