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. 2014 Mar 12;34(11):3947–3958. doi: 10.1523/JNEUROSCI.4013-13.2014

Figure 6.

Figure 6.

PHA-4 regulates synthesis of 5-HT and DA specifically in late adulthood of C. elegans. A, Age-dependent changes in expression of BAS-1 in Pbas-1::bas-1::gfp, daf-2(e1370);Pbas-1::bas-1::gfp and eat-2(ad1116);Pbas-1::bas-1::gfp transgenic worms. Data are from three independent experiments (30 worms per age/genotype). B, Changes in BAS-1 expression level in NSM neurons of eat-2(ad1116);Pbas-1::bas-1::gfp;Punc-119::sid-1 transgenic worms after treatment with dsRNA targeting bas-1. Scale bar, 20 μm. C, Changes in BAS-1 expression level in NSM neurons of eat-2(ad1116);Pbas-1::bas-1::gfp;Punc-119::sid-1 transgenic worms after fed with bacteria expressing dsRNA targeting longevity genes for 4 and 9 d. Approximately 50 worms per RNAi treatment were assayed in five independent experiments. D, Changes in BSR (left) and ESR (right) behaviors of eat-2(ad1116);Punc-119::sid-1 transgenic worms after fed with bacteria expressing dsRNA targeting bas-1 or pha-4. Approximately 33 worms per condition were assayed in three independent experiments. E, Changes in BAS-1 expression level in NSM neurons of eat-2(ad1116);Pbas-1::bas-1::gfp;Punc-119::sid-1 transgenic worms after fed with bacteria expressing dsRNA targeting superoxide dismutase or catalase genes for 4 and 9 d. Data are from ∼50 worms per RNAi treatment in five independent experiments. Worms began to be subjected to RNAi at L4 stage. F, Effects of paraquat on the BAS-1 protein level in young Pbas-1::bas-1::gfp transgenic worms. G, Effects of paraquat on the TPH-1 protein level in young Ptph-1::tph-1::gfp transgenic worms. H, Effects of paraquat on the BAS-1 protein level in eat-2(ad1116);Pbas-1::bas-1::gfp transgenic worms. F–H, Worms at day 1 or day 2 of adulthood were treated with paraquat, and the BAS-1 and TPH-1 level was analyzed 3 d after treatments. More than 30 worms per condition were examined in at least three independent experiments. I, Effects of DTT on the BAS-1 protein level in aged Pbas-1::bas-1::gfp transgenic worms. Worms at day 6 adulthood were transferred to OP50-seeded plates containing DTT, and the BAS-1::GFP level was analyzed 3 d after treatment. Approximately 30 worms per concentration were tested in three independent experiments. Data are mean ± SE. *p < 0.05 (unpaired Student's t test). **p < 0.01 (unpaired Student's t test). ***p < 0.001 (unpaired Student's t test).