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. 2014 Mar 12;34(11):4027–4042. doi: 10.1523/JNEUROSCI.4677-12.2014

Figure 7.

Figure 7.

MAP1B expression is attenuated in SRF-deficient neurons. A, Lack of expression of MAP1B in SRF-deficient neurons. SemiqRT-PCR of total RNA isolated from brains of Srf-NestinCKO mice and Srf-f/f control littermates (n = 3). RPS29 expression served as the control. B, MAP1B expression partially promotes axon growth in SRF-null neurons. Hippocampal neurons from neonatal Srf-NestinCKO mice were transfected with either control myc vector or myc-tagged MAP1B along with farnesylated GFP (F-GFP) and grown for 4 d on poly-d-lysine and laminin. MAP1B was able to partially increase axonal length in SRF-deficient cells. However, MAP1B was not efficient in rescuing the enlarged growth cone (arrows) and abnormal cell soma (arrowheads) morphologies observed in SRF-deficient neurons. Expression of MAP1B did not increase axonal growth in WT neurons. Scale bar, 50 mm. C, Quantitation of axon length and growth cone area from B. ***p < 0.001 (one-way ANOVA and Tukey post-test analysis). n.s., Not significant.