Figure 5.

bAP-evoked Ca²⁺ transients are independent of T-type Ca²⁺ channels in mitral cells. A, NNC 55–0396 (50 μm) raises threshold (left; horizontal lines; 1, pre-NNC; 2, 9 min NNC; 3, 22 min NNC) and attenuates AP amplitude (right; overlaid at the AP generation threshold). B, Representative overlaid traces of somatic APs (threshold at horizontal line) and summary data indicate that the somatic AP shape is unchanged by Z941. Column charts represent the width at half-the threshold to peak amplitude of somatic APs in control (black) and in the presence of Z941 (10 μm; gray) delivered either at −80 (top; n = 5) or −60 mV (bottom; n = 5). C, bAPs (20 Hz, top) were delivered to mitral cells at V_hyper, and Ca²⁺ transients were recorded in the apical tuft in control (black) and in the presence of Z941 (gray). See location in the schematic drawing. Fitted single exponential curves indicate τ_decay. Integrals of Ca²⁺ transients were measured as Amp_total × τ_decay (n = 5, 5–20 min application).