Skip to main content
. 2014 Jan 22;34(4):1370–1379. doi: 10.1523/JNEUROSCI.2440-13.2014

Figure 6.

Figure 6.

Accelerated programmed cell death of motoneurons in Col25a1 KO mice despite preservation of response to trophic support in vitro. A, Immunostaining of p-c-Jun-positive motoneurons in the brachial spinal cord of apoptosis-rescued (Bax KO) and control mice at E14.5. B, Quantification of p-c-Jun-positive motoneurons indicated in A. Data are mean ± SEM. ***p < 0.001. n = 3. C, Survival rate of primary cultured motoneurons derived from Col25a1+/− (n = 7) and Col25a1 KO (n = 4) mice on an Hb9-GFP background at 3 d in vitro, treated with (+) or without (−) NTFs (mixture of BDNF, CNTF, GDNF). Data are mean ± SEM. ***p < 0.001. *p < 0.05. n.s., Not significant. D, Primary cultured motoneurons derived from Hb9-GFP;Col25a1+/+ or Hb9-GFP;Col25a1 KO mice, treated with (+) or without (−) NTF, immunostained by anti-phosphorylated ERK1/2 (red) and anti-GFP (green).