Table 1.
Number of animals assigned for separate experimental groups and for separate end time points in different experiments
| Groupa | Time point (wk)b | Animal no. | Experimentc | Corresponding figure |
|---|---|---|---|---|
| Sham (n = 23) | 9 | 8 | Behavior (Catwalk) | Fig. 1 |
| 3 | 5 | ELISA (SCS) | Fig. 5 | |
| 3 | 3 | Western blot | Fig. 7A–C | |
| 3 | 7 | ELISA (SEC) | Fig. 11 | |
| PBS (n = 18) | 9 | 8 | Behavior, histology | Figs. 1, Figs. 9, Figs. 10 |
| 3 | 3 | Western blot | Fig. 7A–C | |
| 3 | 7 | ELISA (SEC) | Fig. 11 | |
| SCI (n = 5) | 3 | 5 | ELISA (SCS) | Fig. 5 |
| TP (n = 32) | 10 d | 4 | Histology | Figs. 2, Figs. 4 |
| 3 | 8 | Histology | Figs. 2, Figs. 3, | |
| 5 | 5 | Histology | Fig. 2 | |
| 9 | 8 | Behavior, histology | Figs. 1, Figs. 2, Figs. 3, Figs. 9, Figs. 10 | |
| 3 | 7 | ELISA (SEC) | Fig. 11 | |
| TMT (n = 15) | 9 | 8 | Behavior, histology | Figs. 1, Figs. 9, Figs. 10 |
| 3 | 7 | ELISA (SEC) | Fig. 11 | |
| SCI with TMT (n = 5) | 3 | 5 | ELISA (SCS) | Fig. 5 |
| TP + TMT (n = 32) | 10 d | 4 | Histology | Figs. 2, Figs. 4, |
| 3 | 8 | Histology | Figs. 2, Figs. 3, | |
| 5 | 5 | Histology | Fig. 2 | |
| 9 | 8 | Behavior, histology | Figs. 1, Figs. 2, Figs. 3, Figs. 9, Figs. 10 | |
| 3 | 7 | ELISA (SEC) | Fig. 11 | |
| TP with IgG (n = 6) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
| TP with αBDNF (n = 6) | 5 | 6 | Histology | Figs. 6 |
| TP with αNT-3 (n = 6) | 5 | 6 | Histology | Figs. 6 |
| TP with αIGF-1 (n = 6) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
| TP + TMT with IgG (n = 9) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
| 3 | 3 | Western blot | Fig. 7A–C | |
| TP + TMT with αBDNF (n = 6) | 5 | 6 | Histology | Fig. 6 |
| TP + TMT with αNT-3 (n = 6) | 5 | 6 | Histology | Fig. 6 |
| TP + TMT with αIGF-1 (n = 9) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
| 3 | 3 | Western blot | Fig. 7A–C | |
| Total | — | 184 | — | — |
aGroup designations: Sham, sham-operated (laminectomy only) animals; PBS, injured animals with PBS injection (control for NSC transplantation); SCI, animals with injury alone; TP, animals that received NSC TP at 1 week after injury; TMT, animals with TMT alone (PBS injection without TP); SCI with TMT, injured animals with TMT alone; TP + TMT, animals with a combination of TP + TMT. See the Materials and Methods for detailed group designations. Numbers in parentheses indicate the subtotal number of animals in each group.
bTime point refers to the duration elapsed after injury.
cELISA (SCS), ELISA experiment measuring levels of BDNF, NT-3, and IGF-1 in serum, CSF, and spinal cord parenchyma (epicenter); ELISA (SEC) ELISA experiment measuring levels of BDNF, NT-3, and GDNF in the spinal cord tissue at the epicenter and caudal regions.