Figure 4. THBS2 was a target of miR-106a. MiR-106a inhibitor, NC inhibitor, and miR-106a mimics, NC mimics, THBS2-WT and THBS2-MUT were transfected into HEK293 and TCMK-1 cells. (A) The binding site was predicted by Targetscan website. (B) Relative miR-106a level was tested by qRT-PCR. (C) The target relationship between miR-106a and THBS2 was confirmed by dual luciferase reporter assay. (D) THBS2 expression was detected by western blotting. ** p<0.01; *** p<0.001.
