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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Jun 20;133:199–208. doi: 10.1016/j.yjmcc.2019.06.013

Fig. 6. mTOR inhibition normalizes maximal isometric tension and kinetics of activation in single myofibrils.

Fig. 6.

A. A representative image of a single myofibril preparation attached to the glass micro-tools. B. Cross-sectional area (CSA) of myofibrils in experiments (N=9. 10 and 8 for the wild-type, lamp2e2/e2, and lamp2e2/e2 ; xu015+/−, respectively). C. Schematics of the calcium activation and force re-development traces in single myofibrils during release-re-stretch maneuver (*). Shown are WT, LM and LMT groups; D and E. Rates of calcium activation (kACT) and force re-development (kTR) are both higher in lamp2e2/e2 (LM) and normalized in double mutant (LMT). F. Parameters of the rate of fast exponential relaxation (kEXP) have no difference among three groups. G. Maximal tension is increased in lamp2e2/e2 (LM), but rescued in lamp2e2/e2; xu015+/− (LMT). H. Calcium sensitivity (pCa50) is reduced only in double mutant (LMT). I. Hill coefficient is decreased in both LM and LMT groups. * - p < 0.05 to WT # - p<0.05 to LM group (rescue).