Figure 11.

Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A, B, Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with (A) or without (B) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C, Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. *p < 0.05 vs Ctr-Fc.