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. Author manuscript; available in PMC: 2019 Aug 22.
Published in final edited form as: Oncogene. 2018 Dec 5;38(13):2364–2379. doi: 10.1038/s41388-018-0584-6

Fig. 2.

Fig. 2

DHS induces DNA damage and inhibits cell cycle. a Cell-cycle arrested by treatments of DHS or resveratrol at 10 μM for 24 or 36 hours. b FACS to detect BrdU incorporation in DHS-treated U2OS cells. c Cells were harvested after treated with DHS, resveratrol, and analog1 to 5 for 20 minutes for Western blotting for indicated antibodies. d Modified comet assay with alkaline single cell electrophoresis to detect double strand break induced in DHS treated cells. Right panel is the quantification of cells with tail moment. e Immunostaining to examine γH2AX in DHS-treated U2OS cells. Lower panels are the quantification of the immunostaining results and Western blotting for γH2AX. f Annexin V staining for flow cytometry to analyze populations of apoptotic cells in HCT116 cells treated with DHS or resveratrol.

HHS Vulnerability Disclosure