Fig. 5.

DHS interacts with RRM2 at the ferritin-like superfamily domain. a Computational docking of interaction of DHS and RRM2. b The scheme of RRM2 protein domains. The ΔRRM2 contains a deletion from Val146 to Ala167. c The conserve alignment of RRM2 proteins from various species. d Cells were harvested after transfected Flga-RRM2 or Flag-ΔRRM2 plasmid DNA then treated with DHS for Western blotting for Flag-tag proteins and RRM1. e Cellular thermal shift assay to analyze DHS (10 μM) binding with Flag-RRM2, Flag-ΔRRM2, or Flag4A-RRM2. Right panel is the charts of percentages of non-denatured protein fraction.