Skip to main content
. Author manuscript; available in PMC: 2019 Aug 22.
Published in final edited form as: Oncogene. 2018 Dec 5;38(13):2364–2379. doi: 10.1038/s41388-018-0584-6

Fig. 6.

Fig. 6

DHS promotes RRM2 degradation through proteasome degradation pathway. a Cells treated with 10 μM MG132 for 1 hour followed by 48-hour incubation with 10 μM DHS for Western blotting for RRM1 and RRM2. b Western blotting to detect RRM1 and RRM2 protein in RRM1 or RRM2 depleted cells. siGL2 is the control. c Cells were transfected with Flag-RRM2 and HA-Ubiquitin. After treated with 10 μM MG132 for 1 hour followed by 48-hour incubation with 10 μM DHS, proteins were immunoprecipitated with Flag beads for Western blotting for ubiquitin, RRM2, and Flag. d Western blotting to detect RRM1 and RRM2 protein amount in cyclin F knockdown cells. e Cell viability of DHS in RRM2-depleted cells. f Proteins were immunoprecipitated with Flag beads from Flag-RRM2 overexpressed cells treated with DHS for Western blotting for RRM1 and Flag.

HHS Vulnerability Disclosure