Figure 3.

Blocking NKCC1 activation with bumetanide prevented neurite outgrowth of NF200+ (myelinated) neurons. A–C, Confocal images of DRG-dissociated cultures (A, B) and quantification of neurite outgrowth (C) in vehicle-administered (DMSO) or bumetanide-administered cultures. Bumetanide administration induced a decrease in the amount of neurite outgrowth at both concentrations tested (10 and 50 μm) compared with the vehicle (*p < 0.05 and ***p < 0.01, respectively). D, Morphometric analysis of the size of NF200+ neuronal soma and (E) mean soma size for each administered group of cultures. Scale bar, 100 μm. Data are presented as the mean ± SEM.