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. 2015 May 13;35(19):7332–7348. doi: 10.1523/JNEUROSCI.2174-14.2015

Figure 9.

Figure 9.

NCX1 is epigenetically modulated by Sp3/REST/HDAC1/2 complex in tMCAO and by Sp1/HIF-1/p300 in PC+tMCAO. a, ChIP analysis with anti-H3 acetyl of ncx1-Br in CTL and in tMCAO after intracerebroventricular injection of siCTL, siSp3, siREST, siREST+siSp3, and siHDAC1+siHDAC2. IgG was used as negative control. Each column represents the mean ± SEM (n = 5 animals for each column). *p < 0.05 versus CTL. **p < 0.05 versus all. b, c, qRT-PCR and representative WB with quantification of NCX1 in CTL and in tMCAO after intracerebroventricular injection of siCTL, siSp3, siREST, siREST+siSp3, and siHDAC1+siHDAC2. Each column represents the mean ± SEM (n = 6 animals for each column). *p < 0.05 versus CTL. **p < 0.05 versus all. d, ChIP analysis with anti-Sp3 (black bars) and anti-REST (gray bars) of ncx1-Br in CTL and in tMCAO after intracerebroventricular injection of siCTL, siSp3, siREST, siREST+siSp3, and siHDAC1+siHDAC2. IgG was used as negative control. Each column represents the mean ± SEM (n = 6 animals for each column). *,^p < 0.05 versus CTL. *,^^p < 0.05 versus all. e, ChIP analysis with an anti-H3 acetyl of ncx1-Br in CTL and in PC+tMCAO after intracerebroventricular injection of siCTL, siSp1, siHIF-1, siHIF-1+siSp1, and sip300. IgG was used as negative control. Each column represents the mean ± SEM (n = 6 animals for each column). *p < 0.05 versus CTL. f, g, qRT-PCR and representative WB with quantification of NCX1 in CTL and PC+tMCAO after intracerebroventricular injection of siCTL, siSp1, siHIF-1, siHIF-1+siSp1, and sip300. Each column represents the mean ± SEM (n = 6 animals for each column). *p < 0.05 versus CTL. h, ChIP analysis with anti-Sp1 (black bars) and anti-HIF-1 (gray bars) of ncx1-Br in CTL and in PC+tMCAO after intracerebroventricular injection of siCTL, siSp1, siHIF-1, and siHIF-1+siSp1. IgG was used as negative control. Each column represents the mean ± SEM (n = 5 animals for each column). *,^p < 0.05 versus CTL. *,^^p < 0.05 versus all.