Movie 1.
Sequential order of sensory neuron differentiation. SPIM time-lapse analysis of Tg[Isl3:GFP] embryos injected with lyn-TdTomato mRNA to visualize differentiated neurons. Note that the order of neuronal differentiation is sequential, TGg/SAg/LLg; sensory neurons from the ALLg differentiate a bit later (21.0 hpf) and more anteriorly (white arrowhead) than those from the SAg primordium (19.3 hpf). Then, ALLg cells migrate close to the SAg where they rest for a while, before complete segregation of the ganglia is accomplished. First PLLg differentiated cells (yellow arrowhead) are visible at 21.2 hpf when ALLp/SAp are already entering the hindbrain (white arrow). PLLp enters the hindbrain at ∼22.8 hpf (yellow arrow). Embryos were imaged from 19 hpf onward at 5 min intervals. Dorsal view with anterior to the left.