Movie 2.

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DOI: 10.1523/JNEUROSCI.3743-14.2015.video.2

Dynamics of differentiation of TGg, ALLg, and SAg neurons. SPIM time-lapse analysis of Tg[neuroD:GFP] embryos injected with lyn-TdTomato mRNA to see differentiating neurons. Note that ALLg/SAg/PLLg neurons are already differentiating at 16 hpf (white and yellow arrowheads for ALLg and PLLg, respectively). At 18.7 hpf, the projection of the ALLg/SAg (white arrow) reaches the hindbrain; meanwhile the PLLp does so a bit later (20.1 hpf; yellow arrow). The PLLp enters at 22.6 hpf (see it better in the right side of the embryo). The PLLp from the right embryonic side runs anteriorly at 26.4 hpf (yellow arrow), and the PLLp from the left side does so at 29.2 hpf (yellow arrow). Embryos were imaged from 16 hpf onward at 5 min intervals.