Figure 4.

Parthenolide targets axonal growth cones. A, Representative pictures of cultures of adult DRG neurons in two-compartment chambers. Axons regrown beyond the microchannels 24 h after axotomy were visualized using βIII-tubulin immunostaining. The 5 nm par was applied either to the somal (blue) or the axonal (brown) compartments, as indicated in the schematic below the pictures. Scale bar, 250 μm. B, Quantification of axon regrowth beyond microchannels of two-compartment chambers 24 h after axotomy as depicted in A. Data are mean ± SEM from five independent experiments. Treatment effect: **p ≤ 0.01.