Figure 2.

PRPK negatively regulates axon elongation. A, Cultured hippocampal neurons transfected with GFP (control), GFP/PRPK WT, or GFP/PRPK KD. Cells were fixed and immunostained after 3 DIV. Tuj1 staining is also shown. Scale bar, 50 μm. B, Quantification of axon length in cells transfected as in A. n = 50 neurons from three different cultures; one-way ANOVA with Dunnett's post-test (**p < 0.01, ***p < 0.001). C, Quantification of minor neurite length in cells transfected as in A. n = 50 neurons from three different cultures; one-way ANOVA with Dunnett's post-test (no statistically significant differences were observed).