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. 2016 Jul 13;36(28):7392–7406. doi: 10.1523/JNEUROSCI.4061-15.2016

Figure 2.

Figure 2.

Serotonin inhibits TIDA neurons via a postsynaptic mechanism. A, Current-clamp trace, oscillating TIDA neuron. The 5-HT (10 μm)-induced hyperpolarization endures in the presence of TTX (500 nm). Initial membrane potential was set at ≈−65 mV, a value achieved with the injection of negative DC current. Input resistance was assessed by square-form negative current injection (asterisks). For purposes of comparison at peak of response, membrane potential was returned to −65 mV with additional DC current (arrow). Inset, Superimposed membrane potential deflections in control (green) and 5-HT (red). Calibration: 10 mV, 20 s; Inset, 20 mV, 250 ms. B, Summary of 5-HT-induced effects on membrane potential (green) and input resistance (red) recorded as in A. Thick lines indicate mean ± SEM. Dashed lines indicate raw data (n = 5). C, Desensitization of the 5-HT response recorded as in A following repeated applications (after 15 min of wash). Thick lines indicate mean ± SEM. Dashed lines indicate raw data (n = 5). *p < 0.05. **p < 0.01. ***p < 0.005. ns, Not significant.