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. 2016 Jul 13;36(28):7392–7406. doi: 10.1523/JNEUROSCI.4061-15.2016

Figure 9.

Figure 9.

Fluoxetine's effects upon TIDA oscillation and repetitive discharge are independent of 5-HT1A receptor activation and intracellular dialysis. A, Voltage-clamp trace of an oscillating TIDA neuron filtered at 1 Hz. Application of 100 μm fluoxetine continues to abolish TIDA oscillation despite the continuous presence of the 5-HT1A receptor antagonist, NAD299. Calibration: 10 pA, 60 s. Inset, Enlarged areas of A denoted by color. Calibration: Inset, 10 pA, 2 s. B, Fluoxetine continues to inhibit TIDA neuron's capacity for repetitive discharge, reducing the number of APs in response to depolarizing current injection. Box plot organized as Figure 3Bii (n = 8). Right, Current-clamp traces showing the voltage response and AP discharge elicited by injection of a fixed-amplitude positive current pulse during control (green) and during application of fluoxetine (25 μm). Calibration: 20 mV, 50 ms. C, Cell-attached recording of an oscillating TIDA neuron. Fluoxetine (100 μm) continues to abolish the TIDA oscillation when intracellular contents remain undisturbed. Calibration: 10 pA, 2 min. D, Oscillating TIDA neurons patch clamped in whole-cell configuration following preincubation (30 min) with 25 μm fluoxetine (red) also exhibit reduced capacity for repetitive discharge, compared with TIDA neurons that have not been exposed to fluoxetine (green). Box plot organized as Figure 3Bii (n = 6). Right, Traces organized as in B. Calibration: 20 mV, 50 ms. E, Voltage-clamp trace of oscillating TIDA neuron. The 60 min of prolonged recording and intracellular dialysis fails to abolish the TIDA oscillation. Calibration: 20 pA, 2 min. Inset, Expanded areas of E denoted by color, filtered at 1 Hz. Calibration: Inset, 20 pA, 10 s. F, Current-clamp recording of an oscillating TIDA neuron at t = 0 and t = 60 min. The 60 min of prolonged recording fails to abolish TIDA neuron's capacity for repetitive discharge. Calibration: 20 mV, 10 s. Inset, Enlarged areas of F overlaid with trace filtered at 1 HZ according to color. Calibration: Inset, 20 mV, 1 s. ***p < 0.005.