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. 2016 Jul 13;36(28):7407–7414. doi: 10.1523/JNEUROSCI.3065-15.2016

Figure 1.

Figure 1.

Imaging of genetically encoded calcium indicators expressed in DG granule cells of mouse hippocampus. A, Schematic illustration of experimental setup showing the chronic window implant above the intact corpus callosum and area CA1 of the hippocampus that allows for red and green calcium indicator imaging in the intact DG/hippocampal circuit. B, Coronal section of the fixed brain after in vivo imaging sessions. Note the preservation of hippocampal areas CA1 and CA3 (slight distortion of the brain is due to the removal of the cannula required for the chronic cranial window). C, Selective expression of R-CaMP1.07 (unamplified signal from R-CaMP1.07 protein; magenta) and GCaMP6s (unamplified signal from GCaMP6s protein; green) in DG granule cells was confirmed by colabeling with nuclear DAPI (gray, top) and the granule-cell-specific transcription factor Prox1 (cyan, bottom). Scale bars: B, 1 mm; C, top, 50 μm; bottom, 20 μm.