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. 2016 Oct 5;36(40):10472–10486. doi: 10.1523/JNEUROSCI.1174-16.2016

Figure 7.

Figure 7.

Cholinergic sprouting enhanced cholinergic drive in dentate mML in ChAT-ChR2 mice. A, Microphotograph showing the recording/local stimulation zone in the dHPC (top) and the stimulating zone in the MSDB (bottom). Scale bar, 500 μm. Red, Dil embedding of silicon probe (top) and optical fiber (bottom); blue, DAPI staining; green, YFP staining. B, Baseline LFP properties: partial EC lesion did not change total power of LFP, relative theta or gamma power, or oscillatory strength. C, Optogenetic stimulation (5 Hz for 0.5 ms) of cholinergic neurons for 30 s in the MSDB induced a marked increase in theta-band power in the DG mML of lesioned mice, but not in sham mice. D, Dentate mML theta-band power increased during optical stimulation in lesioned mice, but not in sham mice. Dashed lines indicate SEM. E, F, Compared with sham mice, the presence of a cholinergic sprouting in 30 dpl mice increased the strength of the modulation of MSDB cholinergic neurons on dentate mML theta-peak power. Local optical stimulations in the dHPC led to similar effects but with a lower magnitude. *p < 0.05, Newman–Keuls; **p < 0.01, Newman–Keuls; ***p < 0.001, Newman–Keuls.