Figure 6.

Neuronal dystroglycan is not necessary for clustering of glutamatergic synaptic proteins. A, B, To assess integrity of glutamatergic postsynaptic structures, antibodies to PSD-95 and bassoon were used and immunofluorescence quantified in stratum pyramidale and stratum radiatum. Cluster density and size were analyzed in stratum pyramidale and fluorescence intensity in stratum radiatum. All parameters analyzed did not differ substantially between genotypes. C, VGluT1 was used as a marker of glutamatergic presynaptic terminals, and puncta density and size in stratum pyramidale were quantified. No changes in VGluT1 puncta density and size were found between genotypes. D, PSD-95 apposition to VGluT1 was examined in stratum pyramidale and represented as percent PSD-95 clusters apposed to VGluT1 puncta. The apposition of PSD-95 to VGluT1 did not differ between genotypes. Data points represent individual mice (for statistical tests, see Table 2). *p < 0.05. ***p < 0.001.