Table 1.
Antibodies used for immunohistochemical stainingsa
| Target | Host species | Dilution | Catalog # | Company/origin |
|---|---|---|---|---|
| α-Dystroglycan (VIA4–1) | Mouse | 1:100 | 05-298 | EMD Millipore |
| α-Dystroglycan (11H6C4) | Mouse | 1:100 | 05-593 | EMD Millipore |
| β-Dystroglycan | Mouse | 1:100 | ab49515 | Abcam |
| Bassoon | Mouse | 1:2000 | VAM-PS003 | StressGen |
| CB1 | Rabbit | 1:3000 | 258003 | Synaptic Systems |
| Cholecystokinin 8 | Mouse | 1:1000 | ab37274 | Abcam |
| Cre recombinase | Rabbit | 1:1000 | PRB-106C | Covance |
| Dystrophin (C-terminal) | Mouse | 1:100 | BT39-9050-05 | Biotrend |
| GABAAR α1 subunit | Guinea pig | 1:20,000 | — | Fritschy and Mohler, 1995 |
| GABAAR α2 subunit | Guinea pig | 1:6000 | — | Fritschy and Mohler, 1995 |
| GABAAR γ2 subunit | Guinea pig | 1:10,000 | — | Fritschy and Mohler, 1995 |
| GAD65/67 | Rabbit | 1:2000 | GC3008 | Biomol |
| Gephyrin | Mouse | 1:1000 | 147021 | Synaptic Systems |
| NeuN | Mouse | 1:1000 | MAB377 | Chemicon |
| NL2 | Rabbit | 1:10,000 | — | Gift from Dr. Peter Scheiffele |
| PV | Rabbit | 1:1000 | 24428 | ImmunoStar |
| PSD-95 | Mouse | 1:1000 | MA1-045 | ABR |
| Synaptotagmin 2 | Rabbit | 1:1000 | 105123 | Synaptic Systems |
| synArfGEF | Guinea pig | 1:3000 | — | Gift from Dr. Hiroyuki Sakagami (Fukaya et al., 2011) |
| VGAT | Rabbit | 1:3000 | 131003 | Synaptic Systems |
| VGluT1 | Guinea pig | 1:1000 | 135304 | Synaptic Systems |
| VGluT3 | Guinea pig | 1:4000 | AB5421 | Merck Millipore |
aIf not otherwise stated, antibody VIA4–1 was used to label α-dystroglycan. For secondary antibodies, see Materials and Methods.