Figure 1. Binding assays with NF-155– and NF-186–transfected HEK293 cells (A-G) and ELISA (H).

Patients 1, 2, and 3 show a clear binding to NF-155– and NF-186–transfected cells (A–C), whereas patients 4 and 5 only bind to NF-155–transfected and not to NF-186–transfected cells (F and G). Healthy control serum does not bind to the cells (E), and binding of commercial anti–pan-NF autoantibodies to NF-155– and NF-186–transfected cells is shown in (D). NF = neurofascin; OD = optical density.