Figure 6.
Generation and characterization of CB conditional Cbln1-null mice. A, Breeding scheme. CB-Cbln1-null mice were produced by crossing Cbln1flox/flox with Grin2CCre/+ mice. B–E, Immunohistochemical analyses of Cbln1 at adult (3- to 5-month-old; B, D) and juvenile (1-month-old; C, E) CB-Cbln1-null mice. Cbln1 immunoreactivity was significantly reduced in the RSG in addition to the cerebellum in both juvenile and adult CB-Cbln1-null mice. It was also reduced in the Cg in adult CB-Cbln1-null mice. For abbreviations, see the legend to Figure 3. Scale bars, 20 μm for the cerebellum and 500 μm for the rest of the panels. Cbln1 expression levels relative to those in the control mice are shown (B; **p = 5.31 × 10−5; ##p = 3.23 × 10−5; ††p = 7.02 × 10−11; n = 16 where n is the number of images derived from 3–4 animals for each genotype). C, **p = 3.23 × 10−5; ##p = 3.22 × 10−5; n = 12 where n is the number of images derived from 3 animals for each genotype, Mann–Whitney U test).