Figure 4.

Basal mitochondrial oxidative stress is reduced in DMV neurons from A53T-SNCA mice. A, Confocal image of DMV neuron from a wild-type mouse expressing mito-roGFP 2 weeks after viral transfection with the AAV9-CMV-mito-roGFP. B, The relative oxidization was estimated by measuring the resting fluorescence of the mitochondria using 2PLSM (in the somata surrounding the nucleus) relative to post-treatment with strong reducing (2 mm dithiothreitol) and oxidizing (400 μm aldrithiol) agents. Basal mitochondrial oxidative stress is reduced in DMV neurons from A53T-SNCA mice (green boxplot, n = 10 neurons) relative to DMV neurons from wild-type mice (black boxplot, n = 7 neurons). The distribution of relative oxidization in DMV neurons from transgenic mice expressing mito-roGFP under the CMV promoter (dotted gray boxplot; Goldberg et al., 2012) is very similar to the distribution measured from the virally transfected wild-type mice in the present study.