Table 1.
Ratios of long-residence to short-residence component fractions of wild-type CREB and mutant CREB spots with different binding modes (short and long residence time) in cortical neurons
| CREB | Number of cells | Dissociation rate constant (s−1; mean ± SD) |
Ratio of long-residence to short-residence component (mean ± SD) | |
|---|---|---|---|---|
| Short residence | Long residence | |||
| Wild type | 9 | 3.18 ± 0.34 | 0.42 ± 0.13 | 0.120 ± 0.039 |
| L318/325V | 7 | 3.81 ± 0.23 | 0.99 ± 1.37 | 0.043 ± 0.037* |
| R301L | 9 | 3.71 ± 0.15 | 0.77 ± 1.10 | 0.050 ± 0.058* |
The data were evaluated by fitting the distribution histogram of wild-type CREB or mutant CREBs. An asterisk indicates that the ratio of long-residence to short-residence component of mutant CREB (L318/325V or R301L) was significantly different from that of wild-type CREB (*p < 0.05, Mann–Whitney U test with a Bonferroni correction).