Table 2.
TMR-CREB dissociation rate constants in neurons in response to KCl depolarization and TTX
| DIV | Number of cells | Treatment | Dissociation rate constant (s−1; mean ± SD) |
Ratio of long-residence to short-residence component (mean ± SD) | |
|---|---|---|---|---|---|
| Short residence | Long residence | ||||
| 7 | 22 | — | 3.21 ± 0.43 | 0.46 ± 0.07 | 0.124 ± 0.049 |
| 7 | 18 | KCl | 3.02 ± 0.44 | 0.36 ± 0.10** | 0.140 ± 0.059 |
| 14 | 6 | — | 2.74 ± 0.43 | 0.42 ± 0.11 | 0.152 ± 0.043 |
| 15 | 9 | TTX | 3.07 ± 0.43 | 0.46 ± 0.08 | 0.136 ± 0.037 |
The data were evaluated by fitting the distribution histogram of TMR-CREB with or without pharmacological treatments. The asterisks show that the dissociation rate constant of the long component after KCl treatment was significantly different from that in untreated neurons (**p < 0.01, Mann–Whitney U test).