Table 3.
Quantification of synaptic markers in the striatum
| Protein | Mean ± SEM |
|
|---|---|---|
| Gnal+/+ | Gnal+/− | |
| Synaptophysin | 100 ± 22.4 | 86.5 ± 16.7 |
| Gephyrin | 100 ± 18.1 | 91.9 ± 22.1 |
| Homer | 100 ± 7.4 | 102.5 ± 7.3 |
| PSD-95 | 100 ± 12.7 | 102.8 ± 19.9 |
| GluN1 | 100 ± 17.6 | 100.6 ± 18.4 |
| GluN2A | 100 ± 10.8 | 95.6 ± 9.7 |
| GluN2B | 100 ± 14.3 | 97.2 ± 15.4 |
| GluN3A | 100 ± 20.2 | 98.2 ± 12.2 |
| vGlut1 | 100 ± 6.0 | 98.8 ± 9.3 |
| vGlut2 | 100 ± 6.2 | 115.0 ± 8.9 |
Striatal samples from Gnal+/− and WT mice were analyzed by immunoblotting using antibodies against presynaptic and postsynaptic markers as indicated in Table 1. Actin or tubulin immunolabeling was used as a loading control. Data were normalized by actin or tubulin immunofluorescence for each sample and are expressed as a percentage of the mean obtained in WT animals (n = 6–10 per group).