Fig 2. Fusion of 37 primary HIV-1 viruses to single cell suspensions from endometrial biopsies from 3 participants.

Thirty-seven viruses containing BlaM-Vpr were obtained by co-transfection of 293T cells with infectious molecular clones and pcDNA4-3-BlaM-Vpr. Viral supernatants were concentrated and 500 ng p24^Gag was used to infect single cell suspensions generated from EMBs from three participants not using a contraceptive (EMB-3064, EMB-3041 and EMB-3782). The fusion assay was combined with immunostaining and gating as performed in Fig 1. (A) For each of 37 viruses, we display the sampling distribution of the prevalence of immune cells supporting fusion (i.e. % Live CD45⁺BlaM⁺ cells) based on n = 3 participants. Here, the horizontal bar represents the mean while the vertical bar indicates the 95% confidence interval (CI) based on Student’s t-distribution (2 df). (B) Mean (95% CI) fusion susceptibility of 37 viruses grouped by HIV-1 status at the time of viral isolation (founder [TF] vs. chronic [CC]) and subtype (B vs. C). (C) For each participant, distribution of immune cells and fusion susceptibility prevalence by phenotype, averaged over 37 viruses.