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. 2019 Aug 22;15(8):e1007957. doi: 10.1371/journal.ppat.1007957

Fig 4. The effect of dietary Zn on the innate immune response.

Fig 4

Quantitative ELISA analyses of IL-6, in the lungs (A) and blood (D), and IL-1β, in the lungs (B), in Zn-restricted and Zn-replete mice prior to or 36 hrs post infection. The data represent the mean (± S.E.M.) of at least four independent biological replicates with statistical analyses performed using a one-way ANOVA. Transcriptional profiling of the innate immune response for CCL2, in the lungs (C) and blood (F), and IL-1β, in blood (E), in Zn-restricted and Zn-replete mice (n ≥ 6) prior to or 36 hrs post infection by qRT-PCR. Data represent the mean (± S.E.M.) of two independent experiments with statistical analyses performed using a one-way ANOVA. Examination of cell abundances (percentage of live cells of total cells analysed) for alveolar macrophages, in the lungs (G), monocytes, in the lungs (H) and blood (J), neutrophils, in the lungs (I) or blood (K) of Zn-restricted and Zn-replete mice prior to (naïve) or 36 hrs post infection by flow-cytometry (n ≥ 5). Data represent the mean (± S.E.M.) of two independent experiments with statistical analyses performed using a one-way ANOVA.