Figure 1.

Astrocyte-specific overexpression of P2ry1 and astrocyte-specific expression of GCaMP6f. A, ISH of P2ry1. Bottom images, Dark signals represent P2ry1 mRNA. These signals colocalize with GFAP immunostaining, suggesting astrocyte-specific overexpression of P2ry1 (B). C, Astrocyte-specific GCaMP6f expression. Immunohistochemistry for GFAP and GFP (for GCaMP6f). D, Summary of immunohistochemistry. Almost all GFP-positive cells (i.e., GCaMP6f-expressing cells) were GFAP-positive astrocytes.