Figure 3.
Rearing larvae in dim (5 lux) light reduces tectal neuropil growth on 16 dpf compared with larvae reared in standard (100 lux) light (A–C). Scale bar, 40 μm. Rearing larvae in dim light did not affect the number of EdU+ cells generated immediately following a 6 h exposure to EdU in both the medial (D) and lateral (E) neurogenic niches from 5 to 16 dpf. Rearing larvae in dim light reduced the number of EdU+ cells born on 5 dpf in the medial, but not lateral, neurogenic niche by 16 dpf (F–I). Scale bar, 30 μm. Rearing larvae in dim light increased the proportion of activated caspase-3+ cells in the medial, but not lateral, PGZ on 16 dpf (J, K). Lines/bars represent mean ± SEM. *p < 0.05.