Targeted ganglion cells showed the characteristic morphology and light response of tOFF-αRGCs. a, Neurobiotin-injected ganglion cell, showing the characteristic morphology of tOFF-αRGCs with a large soma and dendritic field. Scale bar, 40 μm. ax, Axon. b, Characteristic light response of a tOFF-αRGCs, showing only few spikes during light increment and a transient series of spikes at light decrement (Pang et al., 2003; Van Wyk et al., 2009; Baden et al., 2016; Krieger et al., 2017).