Table 2.

Measures of GLT1 apposition with sleep–wake regulatory neurons by sleep-history condition: 6 h of Rest or 6 h of SD^a

Variable	Rest			SD			p
	Mean	SEM	n	Mean	SEM	n
ORX neurons
Somatic perimeter length (μm)	49.97	0.84	102	52.22	0.82	135	0.060
No. of GLT1 appositions	20.20	0.57	102	21.32	0.53	135	0.153
Mean discrete GLT1 apposition length (μm)	0.96	0.04	101b	0.87	0.02	135	0.025
GLT1 apposition (% somatic perimeter)	39.21	1.48	102	35.24	1.02	135	0.023
MCH neurons
Somatic perimeter length (μm)	47.69	0.89	100	49.07	0.85	111	0.266
No. of GLT1 appositions	17.33	0.63	100	18.25	0.51	111	0.254
Mean discrete GLT1 apposition length (μm)	0.74	0.03	100	0.84	0.04	111	0.027
GLT1 apposition (% somatic perimeter)	27.03	1.23	100	31.34	1.37	111	0.021
ChAT neurons
Somatic perimeter length (μm)	53.34	1.03	100	53.48	0.87	123	0.918
No. of GLT1 appositions	17.87	0.53	100	18.09	0.47	123	0.758
Mean discrete GLT1 apposition length (μm)	0.97	0.04	99b	0.94	0.03	121b	0.498
GLT1 apposition (% somatic perimeter)	32.36	1.25	100	32.11	1.12	123	0.885
PV neurons
Somatic perimeter length (μm)	57.03	2.05	59	55.33	1.68	62	0.521
No. of GLT1 appositions	20.54	1.09	59	22.47	1.29	62	0.260
Mean discrete GLT1 apposition length (μm)	0.99	0.06	59	0.95	0.04	62	0.612
GLT1 apposition (% somatic perimeter)	35.98	2.39	59	37.76	1.91	62	0.559

^aNeurons from 12 rats per behavioral group are included for each neuronal population.

^bOutliers that were >4 SDs from the mean were excluded from analysis.