Figure 7.

Loss of large-caliber axons in Scyl1^−/− and Scyl1^−/−;Scyl3^−/− mice. A, Representative micrograph of toluidine blue-stained semithin sections of sciatic nerve from 4- and 8-week-old WT, Scyl1^−/−, Scyl3^−/−, and Scyl1^−/−;Scyl3^−/− mice. B, Myelinated axon counts in sciatic nerve of 4- and 8-week-old WT, Scyl1^−/−, Scyl3^−/−, and Scyl1^−/−;Scyl3^−/− mice (n = 3 for each age group and genotype). Data are expressed as mean ± SEM. P values, determined by the one-tailed Student's t test, are indicated on the graph. C, Histogram of the frequency of myelinated axons in sciatic nerves of 4- and 8-week-old WT, Scyl1^−/−, Scyl3^−/−, and Scyl1^−/−;Scyl3^−/− mice (n = 3 for each age group and genotype) as a function of axon diameter. Data are expressed as mean ± SEM. P values determined by two-tailed Student's t test. a, WT versus Scyl1^−/−, p < 0.05; b, WT versus Scyl1^−/−; Scyl3^−/−, p < 0.05; c, Scyl1^−/− versus Scyl1^−/−;Scyl3^−/−, p < 0.05.