Figure 2.

c-fos expression in prelimbic cortex, somatosensory cortex, and amygdala after histamine stimulation. A, Mice were subcutaneously injected with histamine (500 μg/50 μl) or vehicle (saline, 50 μl) and c-fos expression was detected in the prefrontal cortex (PrL) (AP∼+1.98 mm), S1/2 (AP∼+0.62 mm), and basolateral amygdala (BLA)/central nucleus (CeA) (AP∼−1.22 mm). B, The PrL was found to have increased c-fos expression after histamine stimulation. Scale bar, 200 μm. PrL: t₍₈₎ = 0.0127. S1/2: t₍₈₎ = 0.05091. BLA/CeA: t₍₈₎ = 0.9999. Data are shown as mean ± SEM. *p < 0.05 compared with vehicle group, Student's t test. n = 5 mice for each group.