Figure 9.

TUNEL labeling and caspase3 immunoreactivity relative to 8OHdG immunoreactivity or EdU defined proliferation status in oligodendrocytes. Percentages of CC1⁺ oligodendrocytes that are TUNEL⁺ at 3 d after injury; comparisons are made between all CC1⁺ cells based on 8OHdG immunoreactivity (a), then between EdU⁻ and EdU⁺ cells, both with elevated 8OHdG (b). Similarly, percentages of CC1⁺ oligodendrocytes that are caspase3⁺ at 3 d after injury based upon 8OHdG immunoreactivity (c), then between EdU⁻ and EdU⁺ cells, both with elevated 8OHdG (d). e, At 28 d, caspase3⁺ oligodendrocyte comparisons are confined to EdU^−/+ as 8OHdG immunoreactivity is not elevated. Each symbol represents the average for an animal. Error bars indicate mean values for the group. Statistical comparisons were made between groups. *p ≤ 0.05, differences in percentages of TUNEL⁺ or caspase3⁺ cells. f, Representative image of caspase3, EdU, CC1, caspase3, EdU and CC1 immunoreactivity at 28 d. CC1⁺/caspase3⁺/EdU⁻ (>) and CC1⁺/caspase3⁻/EdU⁺ (≫) cells are indicated; caspase3⁺/EdU⁺ oligodendrocytes are seldom observed. Scale bar, 20 μm.