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. 2019 Jan 9;11(1):127. doi: 10.3390/nu11010127

Figure 3.

Figure 3

Western blot data showing inhibitory effects of chrysin on AGE-induced cellular induction of phospho-vinculin and phospho-paxillin (A), cortactin and Arp2/3 (B) and fascin-1 and EVL (C) in human renal mesangial cells (HRMC). HRMC were challenged for 3 days with 5.5 mM glucose, 5.5 mM glucose plus 27.5 mM mannitol as osmotic controls, 33 mM glucose or 100 μg/mL AGE-BSA in the absence and presence of 1–20 μM chrysin. db/db mice were orally administrated with 10 mg/kg chrysin for 10 weeks (D). Cellular induction of phospho-vinculin, phospho-paxillin, cortactin, Arp2/3, fascin-1 and Ena/VASP (EVL) were measured by Western blot analysis with each of their primary antibodies. The renal tissue levels of cortactin and fascin-1 were also measured (D). The bar graphs (mean ± SEM, n = 3) in the bottom or right panels represent quantitative results obtained from a densitometer. β-Actin protein was used as an internal control. Values not sharing a letter are different at p < 0.05.