Table 2.
Overview of PTPσ expression constructs
| PTPσ constructs | Amino acid boundary | Oligo sequence (5′-3′) |
|---|---|---|
| PTPσMeA + MeB+ | PTPσ (30-1516) | For pDisplay vector cloning: |
| Forward: GCG AGATCT GAAGAGCCCCCCAGG | ||
| Reverse: GCG GTCGAC TTAGGTTGCATAGTGGTCAAAG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| PTPδ | PTPδ (28-1917) | For pDisplay vector cloning: |
| Forward: GGCCAGATCT CCCGGG GAGACACCCCCCAGGT | ||
| Reverse: CGACCTGCAG CCGCGGC TACGTTGCATAGTGATCAAA | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA CTACGTTGCATAGTGATCAAA | ||
| ΔIg | PTPσ (321-1516) | For pDisplay vector cloning: |
| Forward: GCG AGATCT CTCCCCAAAGCTCCC | ||
| Reverse: GCG GTCGAC TTAGGTTGCATAGTGGTCAAAG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| ΔFN1-2 | PTPσ (30-335/526-1516) | For L-313 vector cloning: 1st fragment |
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTTGGGGAGAGATCTAGATTTCAC | ||
| For L-313 vector cloning: 2nd fragment | ||
| Forward: TCTCCCCAAA TGTACA GTGCCGGGCCAG | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| ΔEcto | PTPσ (856-1516) | For pDisplay vector cloning: |
| Forward: GCG AGATCT GACCCCCAGCCCA | ||
| Reverse: GCG GTCGAC TTAGGTTGCATAGTGGTCAAAG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| ΔCyto | PTPσ (30-890) | For L-313 vector cloning: |
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGTAGAGCAGGATAGCAATGA | ||
| ΔD2 | PTPσ (30-1237) | For L-313 vector cloning: |
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGCCAGCTTCTGGATGTAGGC | ||
| Swap | PTPσ (30-890)/PTPδ (1296-1917) | For L-313 vector cloning: 1st fragment |
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA GTAGAGCAGGATAGCAATGACA | ||
| For L-313 vector cloning: 2nd fragment | ||
| Forward: CCTGCTCTAC TGTACA AAAAGGAAGAGGGCAGAGT | ||
| Reverse: CCGCTTTACT TGTACA CTACGTTGCATAGTGATCAAA | ||
| C1157S | PTPσ (30-1516) | For mutagenesis: |
| Forward: CCATCGTGGTTCACAGCAGTGCCGGTGTG | ||
| Reverse: CACACCGGCACTGCTGTGAACCACGATGG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| R781A | PTPσ (30-1516) | For mutagenesis: |
| Forward: GAGGCGCAGCCTGGCGCACTCGCGTCAG | ||
| Reverse: CTGACGCGAGTGCGCCAGGCTGCGCCTC | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| D1125A | PTPσ (30-1516) | For mutagenesis: |
| Forward: CGGCGTGGCCGGCCCATGGCGTGCC | ||
| Reverse: GGCACGCCATGGGCCGGCCACGCCG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| AAAA | PTPσ (30-1516) | For mutagenesis: |
| Forward: GTCTCAAAGCGCTGAGAGTTGACCGCCGCGCCCGCCGCGTTCCAGGTCACTCGTGGCTTGGG | ||
| Reverse: CCCAAGCCACGAGTGACCTGGAACGCGGCGGGCGCGGCGGTCAACTCTCAGCGCTTTGAGAC | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| R97/100A | PTPσ (30-1516) | For mutagenesis: |
| Forward: CAGCCGCTGGCGACACCGGCGGATGAAAACGTGTACG | ||
| Reverse: CGTACACGTTTTCATCCGCCGGTGTCGCCAGCGGCTG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| Y233S | PTPσ (30-1516) | For mutagenesis: |
| Forward: CACCTGCCAACCTCTCCGTGCGAGAGCTTCG | ||
| Reverse: CGAAGCTCTCGCACGGAGAGGTTGGCAGGTG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC | ||
| R235D | PTPσ (30-1516) | For mutagenesis: |
| Forward: CTGCCAACCTCTACGTGGACGAGCTTCGAGAAGTCCG | ||
| Reverse: CGGACTTCTCGAAGCTCGTCCACGTAGAGGTTGGCAG | ||
| For L-313 vector cloning: | ||
| Forward: ATCCGAATTC GCTAGC ATGGAGACAGACACACTCCT | ||
| Reverse: CCGCTTTACT TGTACA TTAGGTTGCATAGTGGTCAAAGC |