Skip to main content
. 2018 Oct 24;38(43):9202–9214. doi: 10.1523/JNEUROSCI.0876-18.2018

Figure 1.

Figure 1.

Acute modulation of amn expression in adult DPM neurons does not impact memory. A, Constitutive expression of amn-RNAi in DPM neurons leads to a 2 h memory deficit. Flies expressing amn-RNAi1 or amn-RNAi2 under the control of the constitutive VT64246 driver exhibit significantly lower scores than genetic controls (amn-RNAi1: F(2,29) = 6.793, **p = 0.004, n = 10; amn-RNAi2: F(2,39) = 7.415, **p = 0.002, n ≥ 13). B, Constitutive expression of amn-RNAi in DPM neurons leads to a LTM deficit. Flies expressing amn-RNAi1 or amn-RNAi2 under the control of the VT64246 driver exhibit significantly lower scores than genetic controls (amn-RNAi1: F(2,34) = 29.670, ***p < 0.0001, n ≥ 11; amn-RNAi2: F(2,34) = 35.47, ***p < 0.0001, n ≥ 11). C, Immediate memory is not altered by constitutive expression of amn-RNAi in DPM neurons. VT64246/amn-RNAi1 and VT64246/amn-RNAi2 flies show similar scores to genetic controls (amn-RNAi1: F(2,29) = 0.0003, p = 0.100, n = 10; amn-RNAi2: F(2,14) = 0.778, p = 0.481, n = 5). D, amn expression in DPM neurons rescues amnX8 memory defects. amnX8/Y;VT64246/UAS-amn flies expressing amn in DPM neurons show higher MTM scores than their amnX8/Y genetic controls, and similar scores to normal flies expressing amn in DPM neurons (F(3,57) = 13.79, ***p < 0.0001, n ≥ 13). Only males were analyzed. E, Analysis of induced expression of the Gal80ts;VT64246 driver. Gal80ts;VT64246/mCD8::GFP flies were incubated for 3 d at 30°C. Dissected brains were used for immunostaining (green, mCD8::GFP; magenta, nc82). Scale bar, 10 μm. F, Acute inhibition of amn expression in DPM neurons does not impact 2 h memory. After 3 d of induction, amn-RNAi-expressing flies show similar scores to genetic controls (amn-RNAi1: F(2,47) = 1.479, p = 0.239, n = 16; amn-RNAi2: F(2,46) = 2.906, p = 0.065, n ≥ 15). After 5 d of induction, Gal80ts;VT64246/amn-RNAi1 flies show normal 2 h memory (F(2,38) = 0.103, p = 0.903, n = 8). G, Analysis of induced expression of the Gal80ts;VT26149 driver. Gal80ts;VT26149/mCD8::GFP flies were incubated for 3 d at 30°C. Dissected brains were used for immunostaining (green, mCD8::GFP; magenta, nc82). Scale bar, 10 μm. H, Acute inhibition of amn expression for 5 d using the Gal80ts;VT26149 driver does not impact 2 h memory (F(2,38) = 0.119, p = 0.102, n = 8). I, Acute inhibition of amn expression in DPM neurons does not affect LTM. Flies were incubated for 3 d at 30°C before conditioning, and also until memory test. amn-RNAi-expressing flies show similar scores to genetic controls (amn-RNAi1: F(2,24) = 1.693, p = 0.2070, n ≥ 8; amn-RNAi2: F(2,29) = 1.711, p = 0.200, n = 10). J, amn expression in adult DPM neurons does not rescue amnX8 memory defects. After 3 d of induction, amnX8/Y;Gal80ts;VT64246/UAS-amn flies show similar MTM scores to their amnX8/Y genetic controls, and lower scores than normal flies expressing amn in adult DPM neurons (F(3,50) = 3.49, *p = 0.023, n ≥ 12). Only males were analyzed. Error bars indicate SEM.