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. 2018 Oct 24;38(43):9202–9214. doi: 10.1523/JNEUROSCI.0876-18.2018

Figure 4.

Figure 4.

Acute inhibition of amn expression in adult MB neurons leads to memory defects. A, B, amn inhibition in adult MB neurons induces MTM defects. A, Flies expressing amn-RNAi in the adult MB exhibit a 2 h memory deficit (amn-RNAi1: F(2,35) = 7.348, **p = 0.002, n = 12; amn-RNAi2: F(2,39) = 27.81, ***p < 0.0001, n ≥ 13). B, Non-induced flies display normal 2 h memory scores (amn-RNAi1: F(2,29) = 0.822, p = 0.451, n = 10; amn-RNAi2: F(2,34) = 1.242, p = 0.302, n ≥ 11). C, When indicated (4°C), flies were submitted to 2 min cold shock 1 h before the memory test. Cold-shock treatment induces a memory decrease in control flies but not in Gal80ts;238Y/amn-RNAi flies (amn-RNAi1: F(5,75) = 21.99, ***p < 0.0001, n ≥ 12; amn-RNAi2: F(5,71) = 12.98, ***p < 0.0001, n = 12). D, E, amn inhibition in adult MB neurons induces LTM defects. D, Gal80ts;238Y/amn-RNAi-induced flies show LTM impairment (amn-RNAi1: F(2,24) = 35.70, ***p < 0.0001, n ≥ 8; amn-RNAi2: F(2,34) = 20.22, ***p < 0.0001, n ≥ 11). E, In the absence of induction, Gal80ts;238Y/amn-RNAi flies show normal LTM (amn-RNAi1: F(2,29) = 0.393, p = 0.679, n = 10; amn-RNAi2: F(2,29) = 0.363, p = 0.699, n = 10). F, Immediate memory is not affected by amn inhibition in the adult MB (amn-RNAi1: F(2,23) = 0.060, p = 0.942, n = 8; amn-RNAi2: F(2,29) = 0.320, p = 0.729, n = 10). G, LT-ARM is not affected by amn inhibition in the adult MB (amn-RNAi1: F(2,29) = 0.565, p = 0.575, n = 10; amn-RNAi2: F(2,46) = 1.709, p = 0.193, n ≥ 15). H, MTM analyses with the Gal80ts;VT30559 driver. Gal80ts;VT30559/amn-RNAi1 flies show impaired MTM (F(2,35) = 9.953, ***p = 0.0004, n = 12). In absence of induction, Gal80ts;VT30559/amn-RNAi1 flies show normal memory (F(2,35) = 2.547, p = 0.936, n = 12). Error bars indicate SEM.