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. 2018 Oct 24;38(43):9202–9214. doi: 10.1523/JNEUROSCI.0876-18.2018

Figure 6.

Figure 6.

Expression of the wild-type amn gene in MB neurons of amnX8 mutant flies rescues their memory deficits. A, MTM and LTM scores are not affected by expressing amn in the MB. MTM and LTM scores are not affected by expressing amn in the different subsets of the MB with the 238Y driver (MTM: F(2,23) = 5.967, **p = 0.0089, n = 8; LTM: F(2,33) = 1.178, p = 0.3205, n = 12), the c739 driver (MTM: F(2,23) = 0.8268, p = 0.4512, n = 8; LTM: F(2,33) = 0.5209, p = 0.5988, n = 12) or the c747 driver (MTM: F(2,23) = 1.425, p = 0.2629, n = 8; LTM: F(2,33) = 1.419, p = 0.2563, n = 12). B, Analyses with the 238Y driver. amnX8/Y flies expressing amn show higher MTM and LTM scores than their amnX8/Y genetic controls and similar MTM and LTM scores to flies expressing amn in the MB (MTM: F(3,39) = 14.14, ***p < 0.0001, n = 10; LTM: F(3,39) = 10.94, ***p < 0.0001, n = 10). C, Analyses with the c739 driver. amnX8/Y flies expressing amn in the α/β KC display higher MTM and LTM scores than their amnX8/Y genetic controls and similar MTM and LTM scores to flies expressing amn in the α/β KC (MTM: F(3,59) = 66.52, ***p < 0.0001, n = 15; LTM: F(3,59) = 53.87, ***p < 0.0001, n = 15). D, c747 GAL4-driven amn expression does not rescue memory deficits of amnX8 mutant flies. amnX8/Y;c747/UAS-amn flies show similar MTM and LTM scores to amnX8/Y genotypes (MTM: F(3,39) = 11.83, ***p < 0.0001, n = 10; LTM: F(3,23) = 4.449, *p = 0.015, n = 6). Only males were analyzed in these experiments. E, Expression of the wild-type amn gene rescues normal 5HT staining. Canton-S control (n = 8), amnX8/Y mutant (n = 8) and amnX8/Y;c739/UAS-amn (n = 5) brains were analyzed with anti-nc82 and anti-5HT primary antibodies. DPM neuron staining is indicated by a star. Identical Z-stack projections are shown for each panel. A representative brain of each genotype is shown. Scale bar, 10 μm. Error bars indicate SEM.